RBS

Part:BBa_K3766031

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)
Revision as of 08:36, 20 October 2021 by Do-riane (Talk | contribs)

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RBS for TetR in reverse orientation

TetR RBS (reverse orientation)

This part is the TetR RBS in reverse orientation.

Usage and Biology

TetR is a transcriptional regulator inhibited in the presence of tetracycline or anhydrotetracycline [1]. Its expression in reverse orientation (BBa_K3766011) is under the control this RBS and of the TetR/TetA bidirectional promoter (BBa_K3766021 in reverse orientation).

References

[1] Ramos JL, Martínez-Bueno M, Molina-Henares AJ, Terán W, Watanabe K, Zhang X, Gallegos MT, Brennan R, Tobes R. The TetR family of transcriptional repressors. Microbiology and molecular biology reviews: MMBR (2005) 69: 326–356.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None