Translational_Unit
Part:BBa_K3866006
Designed by: Venetios Michelioudakis Group: iGEM21_Thessaly (2021-09-23)
ParaBAD-ackA-Terminator
Usage and Biology
This TU includes the AckA gene placed under the control of the arabinosed-induced promoter. The inducible promoter is used because cloning wasn't done. After the constitutive expression, the metabolism went out of balance so no colonies survied to be chosen for screening.
Design Notes
The coding sequence was domesticated . We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in seva a2 vector and has overhangs compatible for GoldenBraid cloning.
Verification of Cloning
=Experimental Use and Experience
This part showed functionality at this part BBa_K3866009
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1148
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 983
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965
References
- Dittrich, C. R., Bennett, G. N., & San, K. Y. (2005). Characterization of the acetate-producing pathways in Escherichia coli. Biotechnology progress, 21(4), 1062–1067. https://doi.org/10.1021/bp050073s
[edit]
Categories
Parameters
None |