Tag

Part:BBa_K3715034:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-25)
Revision as of 05:53, 25 September 2021 by YuanWang (Talk | contribs) (→‎References)


Sumo tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 212
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 212
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 212
    Illegal BglII site found at 101
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 212
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 212
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We take this one（PETase） as our wild type.


Source

Recently, a novel bacterium Ideonella sakaiensis201-F6 was isolated, which could utilize PET as an energy and carbon source.

References

DOI: 10.1038/s41467-017-02255-z www.nature.com/naturecommunications