![](https://parts.igem.org/images/partbypart/icon_measurement.png)
Measurement
Part:BBa_K3792005
Designed by: Caleb Molinari Group: iGEM21_FSU (2021-09-24)
Revision as of 00:42, 20 October 2021 by Anishshahez (Talk | contribs)
BIOFAB apFAB51 Measurement Device
apFAB51 is a weak promoter taken from the BIOFAB collection. This device uses apFAB51 to express mRFP1, a red fluorescent protein. The apFAB51 test device consists of apFAB51, an RBS, the coding sequence for mRFP1, and a terminator.
This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 614
Illegal AgeI site found at 726 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |