Coding

Part:BBa_K3900002

Designed by: Jonas Dauser   Group: iGEM21_Bielefeld-CeBiTec   (2021-09-21)
Revision as of 16:05, 21 September 2021 by Jodau (Talk | contribs)


Mutated Ribose Binding Protein (RBP) to bind BTCA

This protein was computatinal designed by the IGEM 2021 Bielefeld Team to have a better affinity to Benzenetricarboxylic acid. It is used to iniate a signalling cascade.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 460
    Illegal PstI site found at 676
    Illegal NgoMIV site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None