Part:BBa_K3731003

ppk1-vgb-mazE This part is composed of ppk1, vgb and mazE. From the promoter to the terminator, it express the ppk1, vgb and mazE in sequence. The ppk1 codes PPK1, which can promote the synthesis(major function) and decomposition(minor function) of polyP with the residue of ATP. The vgb can produce HGB so that we can increase the bacteria’s capacity of carrying oxygen, which is beneficial to the growth of bacteria. The mazE codes a kind of antitoxin protein, which can help the bacteria grow longer and produce more polyP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

iGEM2021_Nanjing China Experiment

This year our team develops s polyphosphate kinase (PPK1) overexpression strategy for achieving maximum intracellular polyphosphate accumulation.Through adding vgb and mazE into the E.coli genome, bacteria grows longer and can survive more severe environment, thus increasing the production of polyP.

Reference: Kyunghye Ahn and Arthur Kornberg. Polyphosphate Kinase from Escherichia coli[J]. The Journal of Biological Chemistry, 1990, 265, 20, 11734-11739.