Part:BBa_K3972002

ARG1 (Acoustic Reporter Gene 1)

E. coli codon-optimized ARG1 is a collection of 11 proteins that form gas vesicles when expressed. The proteins involved are GvpA, GvpC, GvpR, GvpN, GvpF, GvpG, GvpL, GvpS, GvpK, GvpJ, GvpT and GvpU.

Usage and Biology

test Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 851
  Illegal EcoRI site found at 4004
  Illegal XbaI site found at 602
  Illegal PstI site found at 3861
  Illegal PstI site found at 3966
  Illegal PstI site found at 4344
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 851
  Illegal EcoRI site found at 4004
  Illegal NheI site found at 736
  Illegal PstI site found at 3861
  Illegal PstI site found at 3966
  Illegal PstI site found at 4344
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 851
  Illegal EcoRI site found at 4004
  Illegal BglII site found at 1355
  Illegal BamHI site found at 4760
  Illegal XhoI site found at 4448
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 851
  Illegal EcoRI site found at 4004
  Illegal XbaI site found at 602
  Illegal PstI site found at 3861
  Illegal PstI site found at 3966
  Illegal PstI site found at 4344
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 851
  Illegal EcoRI site found at 4004
  Illegal XbaI site found at 602
  Illegal PstI site found at 3861
  Illegal PstI site found at 3966
  Illegal PstI site found at 4344
  Illegal NgoMIV site found at 1806
  Illegal NgoMIV site found at 2208
  Illegal AgeI site found at 4727
  
• 1000
  COMPATIBLE WITH RFC[1000]