Coding

Part:BBa_K3784000:Design

Designed by: LUXUAN LIU   Group: iGEM21_BNUZ-China   (2021-09-19)
Revision as of 07:43, 18 October 2021 by MANUL (Talk | contribs) (→‎Design Notes)


acdA (encoding 3-(aryl) acrylate reductase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use gibson recombination to ligate it to the plasmid

Source

Clostridium sporogenes

References