Coding
Part:BBa_K3717006:Experience
Designed by: Enoch Toh Group: iGEM21_TAS_Taipei (2021-09-18)
Revision as of 01:43, 10 October 2023 by Benjamin Ouellet (Talk | contribs) (→Applications of BBa_K3717006)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3717006
iGEM ULaval 2023
We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details.
User Reviews
UNIQ3daa42916b8cb6d4-partinfo-00000000-QINU UNIQ3daa42916b8cb6d4-partinfo-00000001-QINU