Composite
Part:BBa_K3739049
Designed by: Ziyan Han Group: iGEM21_XMU-China (2021-09-09)
J23100-B0030-OmpA-GFP-B0010
This is an anchored proteins onto membranes through OmpA and use GFP to comformation. We use BBa_K3739049 and GFP to verify OmpA' s function which can anchor proteins onto membranes.
Biology
OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with OmpA so that GFP may be displayed on the surface of VnDX.
Characterization
1. Identification
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 494
Illegal SapI.rc site found at 920
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Categories
Parameters
None |