Part:BBa_K4035004

Dimerization of the copper metallothionein 1 : CUP1-(AP)7-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of seven times the AP amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035011). The (AP)7 linker is a common rigid linker made of several successive Alanin followed by Proline.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 90
  Illegal PstI site found at 312
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 90
  Illegal PstI site found at 312
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 90
  Illegal PstI site found at 312
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 90
  Illegal PstI site found at 312
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 306