Part:BBa_K4035003

Dimerization of the copper metallothionein 1 : CUP1-(GGGGS)4-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 ((BBa_M45090), linked together by a flexible linker made of four times the GGGGS amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035010). The GGGGS linker is a common flexible linker.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 90
  Illegal PstI site found at 408
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 90
  Illegal PstI site found at 408
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 235
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 90
  Illegal PstI site found at 408
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 90
  Illegal PstI site found at 408
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 84