Part:BBa_K3739009
LMT-his-hutH
LMT here represents a signal peptide used to secrect the fusion protein outside the cell. The enzyme catalyzes the reaction of converting histidine to form toxic urocanic acid and his-tag is added to purify the protein.
Biology
LMT
Lytic murein transglycosylase (LMT) is an enzyme which is able to degrade murein, a component of cell wall of bacteria. There are two kind of LMTs existing in E.coli: the membrane-binding one and the soluble one. The gene coding LMT homolog is also incorporated in the genome of Vibrio natriegens. The LMT signal peptide (named LMT in our parts) is from the LMT homolog, which can lead the fused protein secreted out of Vibrio natriegens.
hutH
The HutH comes from Pseudomonas putida. Under natural conditions, many microorganisms can use the histidine ammonia-lyase (HutH) to change L-histidine into urocanic acid. HutH catalyzes the first step in the degradation of histidine, and the product urocanic acid is further metabolized to glutamate. This enzyme could be found in the liver of vertebrates and in bacteria such as Escherichia coli, Salmonella and Pseudomonas. It is specific for L-histidine and can be inhibited by D-histidine or imidazole. The active center of the enzyme is thought to be dehydroalanine.
Usage
Used to construct the composite part BBa_K3739040 and BBa_K3739107.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 219
Illegal NgoMIV site found at 655
Illegal NgoMIV site found at 1390
Illegal NgoMIV site found at 1626
Illegal AgeI site found at 292
Illegal AgeI site found at 1119
Illegal AgeI site found at 1615 - 1000COMPATIBLE WITH RFC[1000]
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