Part:BBa_K112245

{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

This part was inserted into the K112995 plasmid between ccdB and the attR2 site.

Performing Gateway in vivo was tested using this part and an RFP part flanked by att sites in an entry vector. Three trials showed very high efficiency(transformations using miniprepped material gave thousands of red colonies), and low co-transformation rate(less than 1%). For more information about this part, visit our [http://2008.igem.org/Team:UC_Berkeley/GatewayPlasmid Wiki!]

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3242
  Illegal PstI site found at 881
  Illegal PstI site found at 5225
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4084
  Illegal PstI site found at 881
  Illegal PstI site found at 5225
  Illegal NotI site found at 141
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 212
  Illegal XhoI site found at 5265
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3242
  Illegal PstI site found at 881
  Illegal PstI site found at 5225
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3242
  Illegal PstI site found at 881
  Illegal PstI site found at 5225
  Illegal AgeI site found at 3674
  Illegal AgeI site found at 3998
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 762
  Illegal BsaI.rc site found at 4366