Regulatory

Part:BBa_K3971000:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-01)
Revision as of 19:51, 1 August 2021 by Misaalbedi (Talk | contribs) (→‎References)


Strong constitutive cyanobacterial PcpcB-7942 mutant promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the sequence as is, since this promoter was created from Synechococcus elongatus PCC 7942 which is a close relative of Synechococcus elongatus UTEX 2973 (1) and their genomes are 99.8% identical (2), thus we used the sequence as is. The sequence did not contain any restriction sites for enzymes we used during cloning as well.

Source

This promoter was generously provided to us by The Wangikar Lab at the Indian Institute of Technology, Bombay.

References

1: Yu, Jingjie, et al. "Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO 2." Scientific reports 5.1 (2015): 1-10.

2:Cui, Jinyu, et al. "Improved salt tolerance and metabolomics analysis of Synechococcus elongatus UTEX 2973 by overexpressing Mrp Antiporters." Frontiers in bioengineering and biotechnology 8 (2020): 500.