Coding

Part:BBa_K108020

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)
Revision as of 10:56, 26 July 2021 by Ng849 (Talk | contribs) (→‎Information contributed by City of London UK (2021))

Dam

DNA adenine methylase of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1]

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3]

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine

[5]


[edit]
Categories
//cds/enzyme/methylation
Parameters
None