Part:BBa_K3425052

Qβ5' recognition sequence of Qβ replicase with RBS

BBa_K3425052 is a construct containing the Qβ5' recognition sequence of Qβ replicase, BBa_K3425038, fused with the RBS designed for Type IIS iGEM Standard assembly, BBa_K3425032.

QB recognition site
References
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 61
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 64
  Illegal XhoI site found at 68
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 61
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 61
  
• 1000
  COMPATIBLE WITH RFC[1000]