Part:BBa_K3425062

β-subunit of Qβ replicase under control of inducible pCAD promoter

BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.

References
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1496
  Illegal XhoI site found at 1004
  Illegal XhoI site found at 1170
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]