Part:BBa_K3600014

Lys2-TRX2pr-mScarleti cassette

This cassette is the product of a two part (BBa_K3600007, BBa_K3600012) Golden Gate BsmBI assembly.

Usage and Biology

This cassette will be integrated into the yeast genome to form a reporter strain that produces a fluorescent signal when the yeast cell is under environmental stress.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1184
  Illegal EcoRI site found at 1914
  Illegal XbaI site found at 1193
  Illegal XbaI site found at 1618
  Illegal XbaI site found at 2091
  Illegal SpeI site found at 3059
  Illegal PstI site found at 3068
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1184
  Illegal EcoRI site found at 1914
  Illegal SpeI site found at 3059
  Illegal PstI site found at 3068
  Illegal NotI site found at 1172
  Illegal NotI site found at 3078
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal suffix found in sequence at 2661
  Illegal EcoRI site found at 1184
  Illegal EcoRI site found at 1914
  Illegal BglII site found at 1636
  Illegal BglII site found at 2532
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1184
  Illegal EcoRI site found at 1914
  Illegal XbaI site found at 1193
  Illegal XbaI site found at 1618
  Illegal XbaI site found at 2091
  Illegal SpeI site found at 3059
  Illegal PstI site found at 3068
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1184
  Illegal EcoRI site found at 1914
  Illegal XbaI site found at 1193
  Illegal XbaI site found at 1618
  Illegal XbaI site found at 2091
  Illegal SpeI site found at 3059
  Illegal PstI site found at 3068
  Illegal NgoMIV site found at 3016
  
• 1000
  COMPATIBLE WITH RFC[1000]