Coding

Part:BBa_K3606809

Designed by: Mingwei Li, Gaochen Zhang   Group: iGEM20_Fudan   (2020-10-24)
Revision as of 02:00, 28 October 2020 by Gao (Talk | contribs)


mcbD

McbD works as synthase responsible for the maturation of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbD into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbG expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbD product was produced.

Results:

More information：https://parts.igem.org/Part:BBa_K3606814


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 374
    Illegal PstI site found at 407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 374
    Illegal PstI site found at 407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 374
    Illegal PstI site found at 407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 374
    Illegal PstI site found at 407
    Illegal NgoMIV site found at 36
    Illegal AgeI site found at 208
  • 1000
    COMPATIBLE WITH RFC[1000]


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