Composite

Part:BBa_K3657035:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 02:25, 28 October 2020 by Liza ch (Talk | contribs) (→‎Design Notes)

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RNA AUAGAU binding Pumby Protein coupled with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
    Illegal XhoI site found at 224
    Illegal XhoI site found at 449
    Illegal XhoI site found at 634
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1337


Design Notes

Proteins were connected with a flexible 3xGS linker.

Source

Synthetic

References