Regulatory

Part:BBa_K3408000:Design

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-02)
Revision as of 01:07, 28 October 2020 by NJAU-Chappie (Talk | contribs) (→‎Source)


promoter nar(fnr regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized.

Source

The sequence is found through the paper 'Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli'.

References

Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli[J]. Applied and Environmental Biology, 2014, 20(02):295-299.