Composite

Part:BBa_K3606114

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 00:59, 28 October 2020 by Gao (Talk | contribs)

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P3 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P3 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.


design:

In this part, the expression of tetR is driven by LuxpR; tetR will block ptetR, resulting in the inability to express McbEFG; and P3 as a constitutive promoter will continue to express McbABCD. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5317
    Illegal PstI site found at 2338
    Illegal PstI site found at 2371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5274
    Illegal PstI site found at 2338
    Illegal PstI site found at 2371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5317
    Illegal PstI site found at 2338
    Illegal PstI site found at 2371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5317
    Illegal PstI site found at 2338
    Illegal PstI site found at 2371
    Illegal NgoMIV site found at 2000
    Illegal AgeI site found at 2172
  • 1000
    COMPATIBLE WITH RFC[1000]


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