Reporter

Part:BBa_K3657018:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 23:47, 27 October 2020 by Liza ch (Talk | contribs) (→‎References)

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dBroccoli Aptamer with added GUAGAG RNA Loop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed as a dimer from the original Broccoli aptamer with the use of DesignSimple in order to exclude the BsmbI cutting site and introduce the GUAGAG loop.

Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly in vitro.

Source

Synthetic

References

Wenjiao S, Strack R L, Nina S, et al. Plug-and-play fluorophores extend the spectral properties of Spinach.[J]. Journal of the American Chemical Society, 2014, 136(4):1198-1201. Filonov G S, Moon J D, Nina S, et al. Broccoli: Rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution.[J]. Journal of the American Chemical Society, 2014, 136.