Composite

Part:BBa_K142203:Design

Designed by: Reine Byun   Group: iGEM08_ETH_Zurich   (2008-10-28)
Revision as of 04:14, 30 October 2008 by Rbyun (Talk | contribs) (→‎Source)

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T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 353


Design Notes

Sequence was optimised to remove Biobrick restriction sites.

Source

The construct was synthesized by Geneart.

References