Composite

Part:BBa_K3396018

Designed by: Huiying Liu   Group: iGEM20_NUDT_CHINA   (2020-10-27)
Revision as of 21:05, 27 October 2020 by Liuhuiying (Talk | contribs)


CMV->Kozak->GFPnano->FKBP->FKBP->P2A ->Truncated TRIM21->FRB->BGH-pA

This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP. And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721


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Categories
Parameters
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