Plasmid

Part:BBa_K3600010

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)
Revision as of 20:45, 27 October 2020 by Yasmine koubaa (Talk | contribs)

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mScarlet-I reporter gene.

mScarlet-I plasmid part was made by LBNC in order to create a part plasmid which contains the mScarlet-I reporter gene and which could be compatible with the Yeast Toolkit.


Usage and Biology

mScarlet-I reporter gene express red fluorescent protein. 1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 914
    Illegal XbaI site found at 1091
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 914
    Illegal BglII site found at 1532
    Illegal BamHI site found at 1661
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 914
    Illegal XbaI site found at 1091
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 914
    Illegal XbaI site found at 1091
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 876
    Illegal BsaI.rc site found at 1668



Measurements

References

[1] 1.Bindels, D. S. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017).

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