Part:BBa_K3331018

E.coli BAD +MazF

This is a suicide switch controlled by an arabinose operon. When arabinose is present, the bacteria activate the suicide switch and die.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2001
  Illegal BamHI site found at 1548
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.

Figure 1:Experimental medium. 6-1 is blank control ,6-4 is experimental group. The protocol of the experiment is list below.