Composite

Part:BBa_K3332087

Designed by: Ruijie Mo   Group: iGEM20_XMU-China   (2020-10-16)
Revision as of 18:44, 27 October 2020 by RuiJie Mo (Talk | contribs) (Usage and Biology)


pTrc-2 derivative-RBS-ECFP-terminator

We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter

With this part, the pTrc-2 derivative promoter can be tested by observing the fluorescence intensity/OD.

Usage and Biology

This part can be used to test that if the pTrc-2 derivative promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 derivative promoter.

Fig 1. pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015)

Characterization

The agarose gel electrophoresis images are shown as below:

caption

Fig 2. pTrc-2 derivative_E0420_pSB1C3[BBa_K3332087] digested by Xba I and Pst I (about 1018 bp)

Reference

[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
abs
biology
colorCyan
directionForward
emissionCFP
emit476
excitation
excite439
kegg
lum
proteinECFP
swisspro
tagNone