Part:BBa_K3630029

Enhanced ferritin expression system

As our BREAC is a microorganism that could tip the balance of the aquarium ecosystem, its removal is another necessary problem that we have to address. Aquariums usually have a filter to clean the water. However, it cannot guarantee the removal of our bacteria. Hence, our design has evolved to include to have the third feature of the project, that is, the magnetization system. When a magnetic field is applied, E. coli can be magnetized and stick on the magnet and it could be cleared.

Constitutively promotes ferritin expression. The ferritin has been linked to iBox-PAK4 which forms crystal structures and enhances the bacteria's sensitivity to magnetic force.

Figure.Magnetization expression construction

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]