Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 10. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 10. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.
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Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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Subunit of phosphonate ABC transporter, phosphonate binding protein, from S.meliloti 1021.Use Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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to construct a new part that can transport glyphosate to cytoplasm.
Biology
Phn system is a gene cluster for organophosphorus transport and degradation in many microorganisms. Sinorhizobium meliloti 1021 encode ABC transporter by phnEE, phnC, phnD gene, this transporter can transport glyphosate to cytoplasm. The phnC gene encodes ATP-binding protein and the phnD encodes phosphonate binding protein of the ABC transporter.
Usage
We ligased the coding part J23100-B0034-phnE1-B0034-phnE2 (Status: 500 Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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) and the part B0034-phnC-B0034-phnD on the expression vector pSB1C3 by standard assembly. Then the ligation mixture was transformed into E. coli DH5α & E. coli BL21(DE3), enabled the E. coli to transport glyphosate to cytoplasm at higher efficiency.
Characterization
Sequence and Features Status: 500 Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.
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