Signalling

Part:BBa_K3482005

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)
Revision as of 17:38, 27 October 2020 by Jeremy95 (Talk | contribs) (→‎Usage and Biology)


NSP4 secretion signal peptide

This part codes for a signal peptide, which can be fused to the N-terminus of a polypeptide in order to export the newly synthesised protein out of the cell.

Usage and Biology

The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in E.coli. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our <E.coli Nissle 1917 strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None