Part:BBa_K3630025
PCB formation LuxR-OmpC dependent expression system
Figure 1. Light dependent and AHL-LuxR dependent express enzyme and adhesion protein)
The figure shows our design for the Light-sensitive LuxR expression and repression system. We use the constitutive promoter (BBa_J23106) to constitutively express LuxR so that under illuminated conditions or red light conditions our LuxR detection system would still be active. Since we want our bacteria to not produce the digestive enzymes when it’s in the dark: We have incorporated a Lac operon (BBa_K3630002) just downstream of the Constitutive promoter (BBa_J23106) for the LuxR expression system. We then put the Lacl (BBa_K3630020) gene under the OmpC (BBa_K3630015) promoter where OmpR would bind. In a dark environment or far-red illumination, our BREAC would express the Lacl (BBa_K3630020) gene and LacI (BBa_K3630020) would bind to the Lac operon (BBa_K3630002) to repress transcription of our LuxR protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1783
Illegal NheI site found at 1806 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4088
Illegal XhoI site found at 2195 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 296
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