Coding

Part:BBa_K3408001

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-03)
Revision as of 16:31, 27 October 2020 by NJAU-Chappie (Talk | contribs)

Phy(ycD)(Phytase)

Phytase is a kind of phosphate-solubilizing enzyme, which can hydrolyze phytate to produce phosphate.

Considering the limitation of pH, we chose the phytase which could work efficiently under pH 6.0 to pH 7.0. We have found the phy(ycD) from Bacillus subtilis YCD whose optimum reaction pH is 6.5(Fig.1). Thus, we constructed phy(ycD) gene into our engineered bacteria.

Fig.1. Effects of pH on the phytase activity (Wang Xi, et al., Applied and Environmental Biology. 2014)


According to the paper, we could see its optimum reaction pH is 6.5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 359
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None