Coding

Part:BBa_K3544003:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)
Revision as of 14:00, 27 October 2020 by Yufengxi (Talk | contribs) (→‎References)

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Coding sequence of BcABA4, a member in biosynthetic gene cluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 696
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 696
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 696
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze α-ionylideneacetic acid into abscisic acid.

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon.

References

Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.