Coding

Part:BBa_K3544003:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)
Revision as of 13:59, 27 October 2020 by Yufengxi (Talk | contribs) (→‎Source)


Coding sequence of BcABA4, a member in biosynthetic gene cluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 696
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 696
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 696
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze α-ionylideneacetic acid into abscisic acid.

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon.

References