Composite

Part:BBa_K1608002

Designed by: Ching-Yuan Chen, Yi-Ru Liu, Pei-Hong Chen   Group: iGEM15_Mingdao   (2015-09-10)
Revision as of 13:37, 27 October 2020 by Alisaleong (Talk | contribs)

GST-SR/pSB1C3

In order to express SR (BBa_K1608000) and SRPK (BBa_K1608001) gene more conveniently, we made two composite biobricks with promoter, RBS and a fusion tag.

Contribution

Team: PuiChing_Macau 2020
Summary: We have compared the original SR without adhesion domains and our engineered SR with adhesion domains, by the fire retardancy test. As shown in figure 1, it is significant that the protein with adhesion domain has a great improvement on its fire retardancy.

<img src="T--Puiching_Macau--bedsheet_chart.png">

Figure 1. Vertical Burning Test using bedsheet

Conclusion

Overall, the proteins with adhesion retain a higher fire retardancy than the protein without adhesion through the fire retardancy test. Therefore, the genetic fusion between SR protein and adhesion domain would generate a more stable fire retardant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1338
    Illegal BglII site found at 1407
    Illegal BglII site found at 1524
    Illegal BamHI site found at 798
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 210


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