Part:BBa_K3033014
FtnA
Derived from E.coli and synthesized through IDT. This codes for the ferratin which would be storing ferric ions for our magnetization function. A gene that originally contained in E.coli, which encodes for ferritin, a globular protein that consist of 24 subunits to form nanocage. The nanocage structure could store Fe3+ ions/Ferritin and therefore enhance the effectiveness and efficiency of E.coli magnetization for movement control.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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No part name specified with partinfo tag. University of Macau 2020 |
UM_Macau 2020: Characterization of Magnetic ability improveDescriptionInspired by the magnetization and control system from UM_Macau team 2019, we design our bacteria cleaning system in the aquarium and make an improvement on their part. They overexpressed ferritin in E.coli to enhance bacterial iron storage capabilities, which allow them to control E.coli movement via magnetization. However, the magnetization capability of Ferritin is poorly limited. We have found literature that proves that tagging this iBox-PAK4 would be enhancing the magnetization capability of the FtnA protein. Ferritin is a ubiquitous iron storage protein. Lager protein assembly that contains more mineralized iron will generate lager magnetic forces. Inka-PAK4, was originally described by Baskaran et. Al, which spontaneously forms needlelike crystals when expressed in mammalian cells. In this literature, researchers found when inkabox with PAK4cat, conformational changes allow the spontaneous crystallization of the complex, producing long rod-shaped crystals with a unit cell that has a hexagonal arrangement of subunits around a hollow channel. Engineered ferritin-containing protein crystals, which named ftn-PAK4, exert magnetic forces that are 9 orders of magnitude larger than those in previous reports.
Figure 1. Schematic of how ferritin subunits fit inside of the crystals’ hollow channel For bacterial expression, Robert C. Robinson has used pGEX4T1 (GE), pET28a (Novagen) and pSY5 (His tagged) as expression vectors for Inka1 and PAK4. Furthermore, the inka-PAK4 and GFP-inka-PAK4 plasmids have been transfected into HEK293T cell and have produced protein crystal successfully. Therefore, we add this iBox-PAK4 encoding gene to the downstream of FtnA in our construct (fig. 2) to reach a stronger magnetic force.
Figure 2. Optimized construct with iBox-PAK4 |
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