Part:BBa_K3629022

mCitrine codon optimized for Yarrowia lipolytica

mCitrine coding sequence codon-optimized for Yarrowia lipolytica.

Usage and Biology

mCitrine is a monomeric green fluorescent protein that is derived from Aequorea victoria. mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm (1). mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. See BBa_K3629026 for a functional high expression construct using this coding sequence.

Design

Below is the codon-optimized sequence of Yarrowia lipolytica mCitrine for use in any expression construct. However, we have also made our own expression construct BBa_K3629026, designed to allow for optimal expression of mCitrine in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 682
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 647
  

References

1. [online] https://www.fpbase.org/protein/mcitrine/ (Accessed October 26, 2020)