![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3447000:Design
Designed by: Jiahao Yu, Xinyue Zhang, Yu Ma Group: iGEM20_Jilin_China (2020-09-03)
luxI, an autoinducer synthetase for AHL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a repetitive sequence between cvaA and cvaB gene. In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.
Source
We get the genomic sequence by NCBI and ordered the part DNA from a synthesis company.