Reporter

Part:BBa_K3526005

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_city   (2020-10-18)
Revision as of 08:36, 27 October 2020 by Lx0402 (Talk | contribs)

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PatzD-EGFP-T7 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The promoter of patzD can sense the presence of atzR protein and express EGFP protein, and then emit green fluorescence.


Contribution

BBa_K3526005 is a bio-sensor that is regulated by the transcription factor PatzD. This part should be used with the part BBa_K3526004, which can consecutively express a transcription factor AtzR. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.

Figure 1
Figure 2

Engineering Success

To test the function of part BBa_K3526005, we co-transform the part BBa_K3526005 and part BBa_K3526004 into Pseudomonas putida KT2440, choose 0μM, 50μM, and 100μM cyanuric acid (CYA) to induce the system. It is found that the reaction has a specificity GFP signal when the concentration of CYA is 100μM.

Figure 3
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Categories
Parameters
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