Device

Part:BBa_K165090

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-29)
Revision as of 04:55, 30 October 2008 by Jszymans (Talk | contribs)

Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306

This is an example of the "R" repressor element in our genetic limiter circuit. It contains the Gli1 and LexA binding sites upstream of mCYC, regulating the expression of the LexA repressor. This is the element of the limiter that represses the gene of interest in the superthreshold state, being induced by G's inducer, the LexA activator in this case. It is repressed by the tau transcription factor when the system is subthreshold, in this case,the Gli1 repressor under control of the threshold-setting promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2587
    Illegal XhoI site found at 1
    Illegal XhoI site found at 122
    Illegal XhoI site found at 284
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal SapI.rc site found at 1758
    Illegal SapI.rc site found at 2351


[edit]
Categories
//chassis/eukaryote/yeast
//function/regulation/transcriptional
Parameters
None