Coding

Part:BBa_K3406003

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-08-27)
Revision as of 06:18, 27 October 2020 by Sorrow-filter (Talk | contribs)

LbaCas13a coding sequence

LbaCas13a is a member of Cas13 protein family.It was originally found in Prevotella sp. MA2016 and has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be activated by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA. Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly A/AC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BglII site found at 3683
    Illegal BamHI site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1738
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 409
    Illegal BsaI.rc site found at 1492
[edit]
Categories
Parameters
None