Project

Part:BBa_J54131

Designed by: Yusaku Nakashima   Group: iGEM06_Tokyo_Alliance   (2006-10-21)
Revision as of 03:42, 27 October 2020 by Ayush2410 (Talk | contribs)

BetI_regulated_promoter-GFP(A-3)

BetI_regulated_promoter-GFP(A-1)

Usage and Biology (added by CCA_San_Diego 2020)

Author: Ayush Agrawal, Noah Zhang

Summary: Provides an overview of the mechanism and its functionality.

Documentation:

Transcriptional factor betI (Betaine inhibitor) is a part of the TetR/AcR family of transcriptional regulators, acting as a repressor of glycine betaine synthesis from choline. BetI repressor protein remains bound to DNA during induction. Exposed to choline, betI forms a complex with DNA binding to a 20-bp-long sequence over betTp and betIp (Santos-Zavaleta, 2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 759
[edit]
Categories
Parameters
None