Composite

Part:BBa_K3338013

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)
Revision as of 00:33, 27 October 2020 by Jonas Scholz (Talk | contribs) (→‎Characterization)

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CMV-EGFP-P2A-mCherry

This part contains an EGFP-P2A-mCherry cassette under control of the constitutively active CMV-promoter. The fluorescent proteins EGFP and mCherry can be independently detected. It was used to compare the functionality of the P2A peptide and an IRES for the expression of two proteins from one single promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

This part was used to characterize the P2A self-cleaving peptide. A detailed documentation can be found on the page of the basic part BBa_K3338003.

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Categories
Parameters
None