Coding

Part:BBa_K3331002

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-20)
Revision as of 21:56, 26 October 2020 by Cycling (Talk | contribs) (→‎Characterization)


E.coli pgmA

This part catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate.

This part comes from the genome of E.coli. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1770bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
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Parameters
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