Part:BBa_K3504017

SP6 Promoter

NOTICE: Parts in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.

Part Description

Sp6 is a constitutive promoter from Sp6 bacteriophage but requires SP6 RNA polymerase When used for in vitro transcription, and according to its orientation the promoter transcribes either the sense OR antisense transcript.

Usage

SP6 promoter controls the transcription of the nSPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells.(1)

References

1-Kohno, A., Emi, N., Kasai, M., Tanimoto, M., & Saito, H. (n.d.). Semliki Forest virus-based DNA expression vector: Transient protein production followed by cell death. Retrieved October 26, 2020, from https://www.nature.com/articles/3300589.pdf?origin=ppub

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]