Plasmid

Part:BBa_K3338020

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)
Revision as of 23:04, 26 October 2020 by Jonas Scholz (Talk | contribs)

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pEGFP-C2

pEGFP-C2 is a commercially available vector for protein expression in mammalian cells with and without a N-terminal EGFP-Tag. It contains a kanamycin/G418 resistance gene for antibiotic selection in bacteria and eukaryotic cells. Furthermore, it exhibits a ColE1 ori and a f1 ori. The EGFP-MCS cassette is under control of an CMV-enhancer/promoter that can be replaced using the AseI-site upstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1363
    Illegal XbaI site found at 1406
    Illegal PstI site found at 1368
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1363
    Illegal NheI site found at 591
    Illegal PstI site found at 1368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1363
    Illegal BglII site found at 1343
    Illegal BamHI site found at 1394
    Illegal XhoI site found at 1347
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1363
    Illegal XbaI site found at 1406
    Illegal PstI site found at 1368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1363
    Illegal XbaI site found at 1406
    Illegal PstI site found at 1368
    Illegal NgoMIV site found at 1976
    Illegal NgoMIV site found at 3259
    Illegal NgoMIV site found at 3542
    Illegal AgeI site found at 600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3754
    Illegal SapI.rc site found at 3108
    Illegal SapI.rc site found at 3318


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