Coding

Part:BBa_K3544000:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-04)
Revision as of 12:46, 27 October 2020 by SCU-XYL (Talk | contribs) (→‎Design Notes)


Coding sequence of BcABA1, a cytochrome P450s belong to biosynthetic genecluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1107


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze alpha-ionylideneethane into alpha-ionylideneacetic aicd

Source

Escherichia coli

References