Composite
Part:BBa_K3396016
Designed by: Changtai Xiao Group: iGEM20_NUDT_CHINA (2020-10-26)
CMV-replaceable-Fluc-P2A-Rluc
Dual luciferase reporter plasmid to indicate the target protein abundance. The coding sequence of target protein can be inserted into the replaceable region with Golden Gate assembly, the ratio Fluc/Rluc shall indicate the normalized protein amount of the target.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 871
Illegal NgoMIV site found at 2215
Illegal NgoMIV site found at 2236
Illegal AgeI site found at 1939 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3102
Illegal SapI.rc site found at 2121
[edit]
Categories
Parameters
None |